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Article: Axonal Segregation and Role of the Vesicular Glutamate Transporter VGLUT3 in Serotonin Neurons.

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Voisin AN; Mnie-Filali O; Giguère N; Fortin GM; Vigneault E; El Mestikawy S; Descarries L; Trudeau LÉ
Front Neuroanat, 2016


Density of SERT, VGLUT3 and SERT/VGLUT3 axon terminals in immature and mature VGLUT3 WT mice.

Dorsal striatum Lateral septum Ventral Hipp Dorsal Hipp
P15 P60 P15 P60 P15 P60 P15 P60
Surface (μm2) 9612 6855.6 9184.8 10173.4 9754.4 7749.6 8828.8 8965.8
SERT 20.8 ± 2.1 18.6 ± 3.7 15 ± 1.7 26.4 ± 4.5 20.4 ± 3.7 26.7 ± 4.6 15 ± 3.6 22.2 ± 4.6
VGLUT3 77 ± 11.4 42.8 ± 5.2 50.4 ± 9.3 32.8 ± 6.2 24.4 ± 1.4 24 ± 7.08 38.8 ± 6.7 38.8 ± 8.2
SERT/VGLUT3 0.2 ± 0.2** 0 2.8 ± 1.2** 4 ± 1.7 6.8 ± 3.4** 3.7 ± 1.4 3.2 ± 1.4** 3.2 ± 1.2

Mean (± SEM) number of immunoreactive axon terminals per surface of thin sections examined in different brain regions after dual immunolabeling in WT mice at immature (P15) or adult (P60–70) age (n = 5 mice per age). Statistical differences were calculated on the ratio of labeled terminals per examined surface. **p < 0.01 compared to VGLUT3 in P15 mice; p < 0.05 compared to VGLUT3 in P60 mice.


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